ELISA
ELISA is a golden standard immunoassay technique used for determination of specific proteins, mostly antibodies. Using the simple principle of antibody-antigen bond, it gives us the possibility to measure the concentration of virtually any analyte.
In TestLine, we focus on Infectious diseases, Autoimmunity and Veterinary assays. The technology offers high throughput and great reliability along with very simple design and ease of use for everyday testing in laboratories of any size and capacity.
Our aim is to make ELISA automation look feasible, user-friendly and affordable. With the help of our SmartKits® designed for Agility® instrument, automation has never been easier.
Key benefits Why is ELISA considered a golden standard?
-
Simple
traditional method with
easy result reading
and interpretation -
Wide portfolio
large variety of products
available -
Ready for automation
broad portfolio
of automated analyzers
suited for our kits -
User-friendly
uniform assay procedure,
color-coded and ready-to-use
components -
Precise
high diagnostic sensitivity
and specificity -
Semiquantitative
and quantitative results
accurate and reliable results,
verified by international
reference materials
Technology How it works?
Sandwich ELISA
Antigen – antibody (from sample) – antibody with HRP (conjugate) format.
Antigens are native, recombinants and or mix of the two. Specific antibodies from the sample bind with antigens during first incubation. All other non-specific antibodies are then washed away.
In the second step, secondary antibodies (which bind to human antibodies from the sample) are added. These antibodies are conjugated with horseradish peroxidase, an enzyme that converts substrate to color product. Reaction is stopped with acidic solution. The intensity of the color is proportional to the concentration of target antibodies from the sample. The color intensity is measured by a photometer at 450 nm.
This is the most used ELISA method across our portfolio.
Capture ELISA
Antibody (capture) – antibody (from sample) – antigen bound to monoclonal antibody conjugated with HRP (conjugate-tracer) format.
Microplate is coated with antibodies specific to human antibodies in the sample. Unbound antibodies are then washed away.
Afterwards, a tracer (specific antigen to targeted antibody bound to another antibody conjugated with HRP) is added. HRP then converts substrate to a color product.
This type of ELISA is used in EIA Toxoplasma kits in IgA, IgE and IgM antibody class to achieve higher sensitivity and specificity.
Antibody Avidity
Avidity is the strength of the non-covalent bond between antigen and antibody. It is typically used for IgG antibodies.
Low avidity antibodies are present during the acute phase of the first infection. They mature to high avidity antibodies over time. High avidity antibodies are typical for reinfections.
With the addition of urea solution, hydrogen bonds within antibody-antigen bonds are disrupted in the case of low avidity antibodies. Strong avidity antibody-antigen bonds persist even after the addition of urea.
Avidity is measured in CMV, EBV VCA, HSV, Measles, Rubella, TBEV, Toxocara and Toxoplasma kits. The avidity solution is always included in the kit and is not required to be bought separately.
ELISA kit
Diagnostic parameters
Complex portfolio
to complement all you need.
- Gastrointestinal infections Discover more
- Herpetic infections Discover more
- Parasitology Discover more
- Respiratory infections Discover more
- Sexually transmitted diseases Discover more
- Post-vaccination panel Discover more
- Tick borne transmitted infections Discover more
- Viral Exanthems Discover more
- Cooperation with NovaTec Immunodiagnostica Discover more
- Extractible nuclear antigens (ENA) Discover more
- Rheumatoid arthritis Discover more
- Coeliac disease and Food intolerance Discover more
- Disease of swine Discover more
- Disease of cattle Discover more
- Disease of dog Discover more
- Disease of fish Discover more
Workflow Simple, yet effective.
-
EIA kit
Automated processing
Manual processing
DYNEX DS2
Compact, practical, and time-saving solution for ELISA automation.
Manufacturer: DYNEX® Technologies, USA
- 2 microplate analyzer
- Open system
- Sample capacity: 100 serum tubes
- Up to 6 different parameters simultaneously
- OD range: 0–3.0
- 2 independent incubation units
- LIS connectivity
- Liquid-level monitoring
- Barcode scanning
- Possibility of two stage dilution
- TestLine Rack – unique insert for TL reagent bottles
- IVDR (2017/746) or IVDD (98/79/EC) compliant
DYNEX DSX
Medium sized, effective, and reliable solution for ELISA automation.
Manufacturer: DYNEX® Technologies, USA
- 4 microplate analyzer
- Open system
- Sample capacity: 96 serum tubes
- OD range: 0–3.0
- 4 independent incubation units
- LIS connectivity
- Liquid-level monitoring
- Barcode scanning
- Possibility of two stage dilution
- TestLine Rack – unique insert for TL reagent bottles
- IVDR (2017/746) or IVDD (98/79/EC) compliant
Gemini
An ideal solution for ELISA processing.
Manufacturer: STRATEC SE, Germany
- 2–3 microplate analyzer
- Open system
- Combined capacity for samples, reagents and controls, e.g: 144 samples + 8 reagents + 16 controls
- OD range: 0–3
- 2 independent incubation units
- LIS connectivity
- Liquid-level monitoring
- Possibility of two stage dilution
- Integrated PC
- IVDR (2017/746) or IVDD (98/79/EC) compliant
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SmartEIA kit
Automated processing
Manual processing
DYNEX Agility® system
High throughput, cutting-edge instrument for full automation of ELISA processing.
Manufacturer: DYNEX® Technologies, USA
- 12 microplate analyzer
- Open system
- Sample capacity: 200 serum tubes
- OD range: 0–3.5
- 12 independent incubation units
- Up to 16 SmartKit® carriers stored on-board or simultaneous runs
- LIS connectivity
- Liquid-level monitoring
- Automatic barcode scanning
- Automatic strip check
- Possibility of two stage dilution
- Continuous sample loading
- Integrated PC
- Supplied with a barcode printer
- IVDR (2017/746) or IVDD (98/79/EC) compliant
TestLine SmartKits®
An innovative way for fully automated ELISA processing.
- Designed for high throughput and automation
- Inserts compatible with DYNEX Agility® system
- Preprinted bar-codes with related assay information (assay type, expiry, volume of tests left, etc.)
- Can also be used for manual processing or with other instruments
- Practical cap holder included
Three easy steps to start testing:
- Remove the caps
- Place the insert into an Agility® rack and the instrument itself
- Run the assay
Dynex Agility® system can process virtually any conventional, ready-to-use ELISA kit, that is meant to be processed on an open platform ELISA instrument. Kits are divided into different categories, depending on the compatibility:
- Gold kit – All TestLine EIA kits, ready-to-use SmartKit®.
- Silver kit* – Kits with reagent bottles that fit into a SmartKit®, but are not supplied as SmartKits®.
- Bronze kit* – Kits with reagents that need to be transferred to SmartKit® compatible bottles.
*For Silver and Bronze kit, SmartKit® inserts are not part of the kit and must be ordered separately.
Evaluation Index of positivity
- simple ratio between sample absorbance and the mean of cut-off values
- semi quantitative
- easy to calculate
- predetermined intervals (0 – 0,9 negative, 0,9 – 1,1 borderline, 1,1 < positive)
Quantitative evaluation
- calculated from the calibration curve
- calibrators included in the kit
- unitage and evaluation is adjusted individually from international standards and reference materials
- may be performed automatically with most commercially available readers
Antibody index - intrathecal synthesis analysis
- software developed by TestLine
- important tool for the determination of neuroborreliosis
- widely accepted Reiber’s formula
- LIS connectivity
- quick and easy evaluation
- validated for Borrelia kits
